Supplementary Fig. S4 from Cancer Cell Fitness Is Dynamic

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Abstract
Supplementary Fig. S4 Tagging of the endogenous locus of Ki67 with EYFP. Sequence coding mVenus-P2A-Neomycin was PCR amplified from the eFlut-mVenus-Neomycin plasmid generously donated by Galit Lahav (Harvard University) and used to insert flanking regions of about 500 nt, amplified with the indicated primers. This was used for homologous recombination in the CRISPR-Cas9 targeted C-terminal region of Ki67 gene.