Molecularly imprinted polymers applied to the clean-up of zearalenone and α-zearalenol from cereal and swine feed sample extracts

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Abstract
A molecularly imprinted polymer prepared using 1-allylpiperazine (1-ALPP) as the functional monomer, trimethyltrimethacrylate (TRIM) as the crosslinker and the zearalenone (ZON)-mimicking template cyclododecanyl-2,4-dihydroxybenzoate (CDHB) has been applied to the clean-up and preconcentration of this mycotoxin (zearalenone) and a related metabolite, α-zearalenol (α-ZOL), from cereal and swine feed sample extracts. The extraction of ZON and α-ZOL from the food samples was accomplished using pressurized liquid extraction (PLE) with MeOH/ACN (50:50, v/v) as the extraction solvent, at 50 °C and 1500 psi. The extracted samples were cleaned up and preconcentrated through the MIP cartridge and analyzed using HPLC with fluorescence detection (λ exc=271/ λ em=452 nm). The stationary phase was a polar endcapped C18 column, and ACN/MeOH/water 10/55/35 (v/v/v, 15 mM ammonium acetate) at a flow rate of 1.0 mL min−1 was used as the mobile phase. The method was applied to the analysis of ZON and α-ZOL in wheat, corn, barley, rye, rice and swine feed samples fortified with 50, 100 and 400 ng g−1 of both mycotoxins, and it gave recoveries of between 85 and 97% (RSD 2.1–6.7%, n=3) and 87–97% (RSD 2.3–5.6%, n=3) for α-ZOL and ZON, respectively. The method was validated using a corn reference material for ZON.

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