Metabolic Engineering of Escherichia coli for the Production of Cadaverine from Lignocellulose

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Abstract
Efficient production of biobased monomers from lignocellulosic hydrolysates is considered an important approach to reducing product costs and achieving sustainable biomanufacturing. However, the low conversion efficiency leads to a low yield and extended fermentation cycles. Here, we enhanced the utilization efficiency of xylose by strengthening the isomerase pathway. Subsequently, we successfully developed an efficient strain capable of synchronous glucose-xylose utilization for cadaverine production, thereby reducing the fermentation time from 30 to 24 h. Through optimization of the fermentation process, cadaverine production reached the highest level of 60.74 g/L in glucose-xylose mixture fermentation, with a yield of 0.34 g/gsugar and a total sugar utilization rate of 3.65 g/L/h in a 5 L fermenter. When applied to the fermentation of corn stalk hydrolysate, 24.42 g/L cadaverine was obtained, with a yield of 0.32 g/gsugar. This work has laid the foundation for the large-scale production of biobased monomers from lignocellulosic hydrolysates.
Funding Information
  • National Key Research and Development Program of China (2024YFA0916800)
  • Natural Science Foundation of Jiangsu Province (BK20231272)
  • National Natural Science Foundation of China (22278218)
  • Jiangsu Basic Research Center for Synthetic Biology (BK20233003)

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