Characterization of pellet morphology during submerged growth of Streptomyces tendae by image analysis

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Abstract
Many filamentous bacteria and fungi tend to form pellets, or mixtures of dispersed mycelium and pellets in liquid fermentation broths. In some cases, a specific kind of morphology is required for optimum product yield. When quantitative analysis and characterization of the pellet morphology are needed, an image processing system can be used. It allows a fast and reproducible analysis of the frequency distribution of pellet size, mean pellet size, contents of pellets, or their shape. The use of such a system allows for an on‐line analysis. For a demonstration of the method, results of two fermentations of Streptomyces tendae are shown.

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