Short-coherence off-axis holographic phase microscopy of live cell dynamics
Open Access
- 22 August 2012
- journal article
- Published by Optica Publishing Group in Biomedical Optics Express
- Vol. 3 (9) , 2184-2189
Abstract
We demonstrate a single-shot holographic phase microscope that combines short-coherence laser pulses with an off-axis geometry. By introducing a controlled pulse front tilt, ultrashort pulses are made to interfere over a large field-of-view without loss of fringe contrast. With this microscope, quantitative phase images of live cells can be recorded in a full-field geometry without moving parts. We perform phase imaging of HEK293 cells, to study the dynamics of cell volume regulation in response to an osmotic shock.Keywords
This publication has 23 references indexed in Scilit:
- Diffraction phase microscopy with white lightOptics Letters, 2012
- Topography and refractometry of nanostructures using spatial light interference microscopyOptics Letters, 2010
- Time-domain optical coherence tomography with digital holographic microscopyApplied Optics, 2005
- Digital recording and numerical reconstruction of hologramsMeasurement Science and Technology, 2002
- Short-coherence digital microscopy by use of a lensless holographic imaging systemApplied Optics, 2002
- Digital in-line holography for biological applicationsProceedings of the National Academy of Sciences, 2001
- Digital holography for quantitative phase-contrast imagingOptics Letters, 1999
- Optical Coherence TomographyScience, 1991
- Two-Photon Laser Scanning Fluorescence MicroscopyScience, 1990
- Reconstructed Wavefronts and Communication Theory*Journal of the Optical Society of America, 1962