Detection of Hybridomas Secreting Monoclonal Antibodies to Human Gamma Interferon Using a Rapid Screening Technique and Specificity of Certain Monoclonal Antibodies to Gamma Interferon

Authors:
  • AMA. Meager
    A. Meager
  • SPS. Parti
    S. Parti
  • SBS. Barwick
    S. Barwick
  • JSJ. Spragg
    J. Spragg
  • KOK. O'HAgan
    K. O'HAgan
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Abstract
An immunoprecipitation technique, which uses radiolabeled human gamma interferon (HuIFN-γ) and a sheep anti-mouse F(ab')2 serum, is described for the rapid detection of mouse monoclonal antibodies to IFN-γ in hybridoma culture supernatants. This technique which can be performed in 4 h detects both neutralizing and nonneutralizing antibodies to IFN-γ. Certain monoclonal antibodies prepared against natural (glycosylated) IFN-γ which neutralized the antiviral activity of natural IFN-γ bound strongly to rDNA (unglycosylated) IFN-γ derived from E. coli, but failed to neutralize its antiviral activity.

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