A detection method for Pseudomonas solanacearum in symptomless potato tubers and some data on its sensitivity and specificity

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Abstract
A detection method is described for latent infections of Pseudomonas solanacearum race 3 in potato. This method is based on extraction of 200 heel ends of potato, followed by screening with an indirect antibody stain (IFAS) and (in IFAS‐positive cases) a pathogenicity test on tomato (PT). Using the method in some sensitivity and specificity tests with more than 600 samples it appears that: (a) its detection level is 104 cells ml‐1 in IFAS and 102— 104 cells ml‐1 in PT; (b) per cent recovery is 0.1–10% (10% at lower contamination levels); (c) false‐positive samples due to cross‐reacting bacteria in IFAS were limited to only 2–3%, using two polyclonal antisera against whole cells of P. solanacearum. The merits of the method in comparison with others (ELISA, selective media) are discussed.

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