ISOLATION OF PURIFIED H AND L POLYPEPTIDE CHAINS FROM GUINEA-PIG GAMMA2-IMMUNOGLOBULIN AFTER MILD REDUCTION

Authors:
  • MLM. E. Lamm
    M. E. Lamm
  • VNV. Nussenzweig
    V. Nussenzweig
  • BBB. Benacerraf
    B. Benacerraf
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  • 1 January 1966
    • journal article
    • research article
Abstract
A method is described whereby biologically active H and L chains, free from each other by immunochemical criteria, can be prepared from guinea pig [gamma] 2-immunoglobulin. The procedure makes use of mile reduction and alkylation in the absence of a denaturing agent, followed by gel filtration in the presence of 4 M guanidine.HCl. Specific binding of antigen by the method of equilibrium dialysis was found only when specific H chains were mixed with L chains, with more activity resulting when the L chains were also specific. Specific binding by antibody H chains alone was not found.

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