Automated Nuclear Lamina Network Recognition and Quantitative Analysis in Structured Illumination Super-Resolution Microscope Images Using a Gaussian Mixture Model and Morphological Processing

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Abstract
Studying the architecture of nuclear lamina networks is significantly important in biomedicine owing not only to their influence on the genome, but also because they are associated with several diseases. To save labor and time, an automated method for nuclear lamina network recognition and quantitative analysis is proposed for use with lattice structured illumination super-resolution microscope images in this study. This method is based on a Gaussian mixture model and morphological processing. It includes steps for target region generation, bias field correction, image segmentation, network connection, meshwork generation, and meshwork analysis. The effectiveness of the proposed method was confirmed by recognizing and quantitatively analyzing nuclear lamina networks in five images that are presented to show the method’s performance. The experimental results show that our algorithm achieved high accuracy in nuclear lamina network recognition and quantitative analysis, and the median face areas size of lamina networks from U2OS osteosarcoma cells are 0.3184 μm2.
Funding Information
  • National Key R&D Program of China (2017YFB0403701)
  • Jiangsu Province Key R&D Program (BE2019682, BE2018667)
  • National Natural Science Foundation of China (61605210, 61675226, 61378090)
  • Youth Innovation Promotion Association of the Chinese Academy of Sciences (2019320)
  • Frontier Science research project of the Chinese Academy of Sciences (QYZDB-SSW-JSC03)
  • Strategic Priority Research Program of the Chinese Academy of Sciences (XDB02060000)

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