Creating a conditional mutation of Wnt‐1 by antisense transgenesis provides evidence that Wnt‐1 is not essential for spermatogenesis
Authors:
- 1 January 1993
- journal article
- research article
- Published by Wiley in Developmental Genetics
- Vol. 14 (4) , 274-281
Abstract
We have used mice transgenic for an antisense construct for Wnt-1 to study the role of this gene in post-meiotic sperm development. The human PGK-2 promoter provided levels of Wnt-1 antisense mRNA in testes in 5 transgenic lines greatly in excess of Wnt-1 mRNA concentrations, and Wnt-1 mRNA levels were greatly decreased in the lines, by 98% in three of them. There was a general correlation between copy number of the insert, levels of antisense RNA, and decreases in mRNA. There was little effect of the antisense transgene on fertility or testicular histology suggesting that normal levels of Wnt- 1 transcript are not essential for spermatogenesis. © 1993Wiley-Liss, Inc.Keywords
This publication has 28 references indexed in Scilit:
- Mice deficient for Rb are nonviable and show defects in neurogenesis and haematopoiesisNature, 1992
- Impaired type II glucocorticoid-receptor function in mice bearing antisense RNA transgeneNature, 1992
- Using Antisense RNA to Study Gene Function: Inhibition of Carotenoid Biosynthesis in Transgenic TomatoesNature Biotechnology, 1991
- Antisense RNA production in transgenic miceSomatic Cell and Molecular Genetics, 1990
- Antisense RNA inhibition of HPRT synthesisSomatic Cell and Molecular Genetics, 1990
- Post-meiotic gene expressionTrends in Genetics, 1990
- Conversion of Normal Behavior to Shiverer by Myelin Basic Protein Antisense cDNA in Transgenic MiceScience, 1988
- A novel mutation in myelin-deficient mice results in unstable myelin basic protein gene transcriptsNeuron, 1988
- Post-meiotic transcription of phosphoglycerate-kinase 2 in mouse testesBioscience Reports, 1985
- Molecular Genetic Analysis of Myelin‐Deficient Mice: Shiverer Mutant Mice Show Deletion in Gene(s) Coding for Myelin Basic ProteinJournal of Neurochemistry, 1985