The Detection of Alkaline Phosphatase Using an Electrochemical Biosensor in a Single-Step Approach
Open Access
- 30 October 2009
- Vol. 9 (11) , 8709-8721
Abstract
A one-step, single use, disposable Alkaline Phosphatase (ALP) biosensor has been developed. It is based on the detection of phenol produced by an ALP enzymatic reaction. It can operate at 25 °C in a pH 10 medium. It measures ALP of 0–300 IU/L. The permissible concentrations of glucose, ascorbic acid and urea without interference are 10 mM/L, 5 mg/L and 400 mg/L, respectively. Experimental results are compared to those obtained by spectrophotometric measurements in bovine serum. Excellent linearity between the biosensor outputs and the ALP concentrations exists. The agreement between the measurements of this biosensor and the spectrophotometer is also outstanding.Keywords
This publication has 16 references indexed in Scilit:
- Determination of total bile acid levels using a thick-film screen-printed Ir/C sensor for the detection of liver diseaseThe Analyst, 2009
- Detection of lipoprotein-associated phospholipase A2 using a nano-iridium particle catalyst-based biosensorSensors and Actuators B: Chemical, 2008
- An electrochemical biosensor of the ketone 3-β-hydroxybutyrate for potential diabetic patient managementSensors and Actuators B: Chemical, 2008
- An iridium nanoparticles dispersed carbon based thick film electrochemical biosensor and its application for a single use, disposable glucose biosensorSensors and Actuators B: Chemical, 2007
- Highly sensitive electrochemical detection of alkaline phosphataseAnalytica Chimica Acta, 2000
- Improved stability and altered selectivity of tyrosinase based graphite electrodes for detection of phenolic compoundsAnalytica Chimica Acta, 1999
- Spectrophotometric Assay for Superoxide Dismutase Based on Tetrazolium Salt 3′-{1-[(Phenylamino)-carbonyl]-3,4-tetrazolium}-bis(4-methoxy-6-nitro)benzenesulfonic Acid Hydrate Reduction by Xanthine–Xanthine OxidaseAnalytical Biochemistry, 1997
- Zeptomole-Detecting Biosensor for Alkaline Phosphatase in an Electrochemical Immunoassay for 2,4-Dichlorophenoxyacetic acidAnalytical Chemistry, 1996
- Amperometric detection of alkaline phosphatase activity at a horseradish peroxidase enzyme electrode based on activated carbon: potential application to electrochemical immunoassayBiosensors and Bioelectronics, 1995
- Comparison of methods for following alkaline phosphatase catalysis: Spectrophotometric versus amperometric detectionAnalytical Biochemistry, 1991