A Simple Two‐step Isolation Procedure for Human and Bovine Antithrombin II/III (Heparin Cofactor): a Comparison of Two Methods
Authors:
- 1 October 1975
- journal article
- research article
- Published by Wiley in British Journal of Haematology
- Vol. 31 (2) , 233-243
Abstract
Summary.: Human and bovine antithrombin II/III have been isolated by a simple procedure essentially using only affinity chromatography on heparin‐agarose and polyethylene glycol precipitation. An additional ammonium sulphate step removes polyethylene glycol and trace contaminants. The final preparation is homogeneous by SDS‐, disc‐ and agar slide electrophoresis. End group analysis of human antithrombin II/III shows histidine as the N‐terminal amino acid. The pure preparation showed both progressive antithrombin activity and heparin cofactor activity.Keywords
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