Changes in ATP and Cyclic Nucleotide Levels during Sympathetic Nerve Stimulation in Canine Subcutaneous Adipose Tissue in situ

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Abstract
Subcutaneous adipose tissue in fed, female dogs was isolated. Biopsies of the tissue (30–150 mg) were taken and rapidly frozen in liquid nitrogen before, during and after nerve stimulation (3–4 Hz). In unstimulated adipose tissue the levels of ATP1were 74 ± 7 nmol/g, of cyclic AMP 90 ± 12 pmol/g and of cyclic GMP 18 ± 3 pmol/g (mean + S.E.). During sympathetic nerve stimulation the levels of ATP and cyclic GMP fell by 30 and 50% respectively (p < 0.01), while the cyclic AMP content increased by 50 % (p < 0.05). After nerve stimulation there was a marked increase in glycerol release, and the levels of all three nucleotides returned to control. The fall in ATP during nerve stimulation was essentially eliminated by prior adrenergic a‐receptor blockade. It is concluded that 1) sympathetic nerve stimulation induces a rapid, reversible fall in tissue ATP content, which may be related to hypoxia secondary to the vasoconstriction, and 2) lipolytic responses to sympathetic nerve stimulationin vivoare preceeded by small increases in the tissue cyclic AMP level, and a 3‐fold increase in the cyclic AMP/cyclic GMP ratio.

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