Preparation of Proliferated Bovine Primary Skeletal Muscle Cells for Bottom-Up Proteomics by LC-MSMS Analysis
- 27 October 2018
- book chapter
- Published by Springer Nature
- Vol. 1889, 255-266
Abstract
This chapter outlines a method for sample preparation for bottom-up proteomics by LC-MSMS analysis of in vitro proliferated bovine primary skeletal muscle cells. The protocol describes the isolation of bovine primary skeletal muscle cells, extraction of proteins, proteolytic digestion of proteins, and desalting of the final peptide samples. The final peptide samples can be analyzed using various LC-MSMS systems after reconstitution in a suitable elution buffer.Keywords
This publication has 7 references indexed in Scilit:
- A spinnable and automatable StageTip for high throughput peptide desalting and proteomicsProtocol Exchange, 2014
- The combination of glycosaminoglycans and fibrous proteins improves cell proliferation and early differentiation of bovine primary skeletal muscle cellsDifferentiation, 2013
- Notch and Wnt Signaling, Physiological Stimuli and Postnatal MyogenesisInternational Journal of Biological Sciences, 2010
- Universal sample preparation method for proteome analysisNature Methods, 2009
- Mass Spectrometry: Bottom-Up or Top-Down?Science, 2006
- Stop and Go Extraction Tips for Matrix-Assisted Laser Desorption/Ionization, Nanoelectrospray, and LC/MS Sample Pretreatment in ProteomicsAnalytical Chemistry, 2002
- SATELLITE CELL OF SKELETAL MUSCLE FIBERSJournal of Cell Biology, 1961