Exo-Arabinanase of Penicillium chrysogenum Able To Release Arabinobiose from α-1,5- l -Arabinan
Open Access
- 1 July 2001
- journal article
- research article
- Published by American Society for Microbiology in Applied and Environmental Microbiology
- Vol. 67 (7) , 3319-3321
Abstract
An exo-arabinanase, designated Abnx, was purified from a culture filtrate of Penicillium chrysogenum 31B by ammonium sulfate precipitation, anion-exchange chromatography, and hydrophobic chromatography. Abnx had an apparent molecular mass of 47 kDa. The enzyme released only arabinobiose from the nonreducing terminus of α-1,5- l -arabinan and showed no activity towards p -nitrophenyl-α- l -arabinofuranoside and α-1,5- l -arabinofuranobiose. Abnx is the first enzyme with this mode of action.Keywords
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