A SIMPLE METHOD FOR OBTAINING INCREASED CONTRAST IN ARALDITE SECTIONS BY USING POSTFIXATION STAINING OF TISSUES WITH POTASSIUM PERMANGANATE
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Open Access
- 1 November 1961
- journal article
- Published by Rockefeller University Press in Journal of Cell Biology
- Vol. 11 (2) , 492-497
Abstract
Epoxy resin appears to be the best embedding material for preserving tissues for electron microscopy. Its low linear coefficient of contraction I ensures that no appreciable distortion can occur owing to polymerization damage or excessive shrinkage (1). This plastic also is exceptionally stable in the electron beam, so that surface damage to the section is slight. Although Araldite blocks are often more difficult to cut than those of methacrylate, the introduction of the diamond knife (2) with its superior cutting abilities overKeywords
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