Supplementary Methods from Antitumor Activity of a Duocarmycin Analogue Rationalized to Be Metabolically Activated by Cytochrome P450 1A1 in Human Transitional Cell Carcinoma of the Bladder

Authors:
  • MSMark Sutherland
    Mark Sutherland
  • JGJason H. Gill
    Jason H. Gill
  • PLPaul M. Loadman
    Paul M. Loadman
  • JLJonathan P. Laye
    Jonathan P. Laye
  • HSHelen M. Sheldrake
    Helen M. Sheldrake
  • NINicola A. Illingworth
    Nicola A. Illingworth
  • MAMohammed N. Alandas
    Mohammed N. Alandas
  • PCPatricia A. Cooper
    Patricia A. Cooper
  • MSMark Searcey
    Mark Searcey
  • KPKlaus Pors
    Klaus Pors
  • SSSteve D. Shnyder
    Steve D. Shnyder
  • LPLaurence H. Patterson
    Laurence H. Patterson
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Abstract
PDF file - 109K, Methodology for analysis of CYP1 gene and protein expression,Detection of DNA-damage induced gamma-H2AX protein expression, and analysis of ICT2700 metabolism in mouse and human liver ex vivo