The White-Rot FungusPleurotus ostreatusTransformant Overproduced Intracellular cAMP and Laccase

Authors:
  • TSTakaiku Sakamoto
    Takaiku Sakamoto
  • YHYoichi Honda
    Yoichi Honda
  • YKYasuyuki Kagotani
    Yasuyuki Kagotani
  • KIKousuke Izumitsu
    Kousuke Izumitsu
  • KSKazumi Suzuki
    Kazumi Suzuki
  • TIToshikazu Irie
    Toshikazu Irie

Abstract
Transformation of Pleurotus ostreatus PC9 with the mutated heterotrimeric G protein alpha subunit (Gα) gene resulted in higher laccase (Lac) activity and intracellular cyclic adenosine monophosphate (cAMP) concentrations as compared to those in wild-type PC9. The transformant also exhibited higher Lac activity than the wild type when cultured in a medium containing known Lac inducers CuSO4 and ferulic acid.

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