Antibiotics are the treatment of choice for bacterial infections, but their loss of effectiveness presents a global public health issue. The threat from antimicrobial resistance not only lies in single-celled bacteria but in communities of bacteria that aggregate and form biofilms. Antibiotic development has primarily focused on single-celled bacteria with limited consideration of the role of biofilms on disease progression. Bacteria in biofilms are up to 1000 times more resistant to antibiotics than their single-celled counterparts causing relapsing infections that are challenging to treat. This thesis investigates strategies to develop the oxazolidinone antibiotic class as biofilm eradication agents using nitroxides.