Clinical application of the serum 1,5‐anhydroglucitol assay method using glucose 3‐dehydrogenase

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Abstract
We attempted to develop a novel serum 1,5‐anhydroglucitol (1,5‐AG) assay kit using glucose 3‐dehydrogenase (G3DH) from Halomonas sp. α‐15 strain. The major advantages of this method are that the 1,5‐AG detection requires a very small amount of G3DH, and the enzyme catalyzes a simple reaction. The analytical performances were acceptable for clinical use operated with a clinical automatic analyzer. The correlation with a commercial assay kit against sera of healthy volunteers was y=0.975x+0.008, r=0.993, Sy∣x=1.32 μg/mL. However, sham‐negative specimens were observed in the validation of this method using specimens from hospital patients. J. Clin. Lab. Anal. 16:299–303, 2002.

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