Biosynthesis of Lecithin by the CDP-Choline Pathway in Liver Microsomes of Rainbow Trout, Salmo gairdneri

Authors:
  • BHB. J. Holub
    B. J. Holub
  • KNK. Nilsson
    K. Nilsson
  • JPJ. Piekarski
    J. Piekarski
  • SSS. J. Slinger
    S. J. Slinger
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Abstract
The enzymatic synthesis of lecithin from CDP-choline (14C) was characterized in microsomal preparations from the livers of rainbow trout (Salmo gairdneri). Optimal activity was obtained with the addition of 1,2-digylceride and Mg++ to the incubation medium. There was a 290% stimulation in cholinephosphotransferase activity when the incubation temperature was raised from 15 to 37 C. The results indicate that the CDP-choline pathway is operative in the formation of lecithin in the liver of rainbow trout. The possible function of this reaction in the acclimation offish to environmental temperature is discussed.