Characterization of a cDNA clone encoding pedal peptide in the terrestrial snail
Authors:
- Dmitry A. PoteryaevDPDmitry A. Poteryaev
- Igor S. ZakharovIZIgor S. Zakharov
- Pavel M. BalabanPBPavel M. Balaban
- Pavel N. UvarovPUPavel N. Uvarov
- Alexander V. BelyavskyABAlexander V. Belyavsky
- 1 November 1997
- journal article
- research article
- Published by Wolters Kluwer Health in NeuroReport
- Vol. 8 (16) , 3631-3635
Abstract
WE report the isolation of a Helix lucorum cDNA clone encoding a precursor of neuropeptides that are closely related to Aplysia and Tritonia pedal peptides (Pep). The predicted propeptide contains 20 copies of the two variants of Helix Pep interspersed with Lys-Arg endopeptidase cleavage sites. Northern blot hybridization revealed multiple Pep-hybridizing species in the Helix CNS RNA. The Pep gene was expressed by several identified serotonergic neurones in pedal and cerebral ganglia, groups of sensory neurones in procerebrum, peripheral neurones in olfactory bulb, mantle and foot, and group of neurones in pedal ganglia presumably involved in locomotion control. Pep mRNA was detected in several neurones at the early stages of nervous system development.Keywords
This publication has 11 references indexed in Scilit:
- Pedal serotonergic neurons modulate the synaptic input of withdrawal interneurons ofHelixInvertebrate Neuroscience, 1995
- LymnaDFamides, a new family of neuropeptides from the pond snail, Lymnaea stagnalisEuropean Journal of Biochemistry, 1993
- In situ hybridization of whole-mounts of Aplysia ganglia using non-radioactive probesJournal of Neuroscience Methods, 1992
- Distribution and characterization of pedal peptide immunoreactivity in AplysiaJournal of Neurobiology, 1990
- Involvement of pedal peptide in locomotion in Aplysia: Modulation of foot muscle contractionsJournal of Neurobiology, 1990
- PCR-based cDNA library construction: general cDNA libraries at the level of a few cellsNucleic Acids Research, 1989
- Sequence of pedal peptide: a novel neuropeptide from the central nervous system of AplysiaJournal of Neuroscience, 1989
- Immunocytological localization of pedal peptide in the central nervous system and periphery of AplysiaJournal of Neuroscience, 1989
- A new method for predicting signal sequence cleavage sitesNucleic Acids Research, 1986
- Translocation of proteins across the endoplasmic reticulum. II. Signal recognition protein (SRP) mediates the selective binding to microsomal membranes of in-vitro-assembled polysomes synthesizing secretory protein.Journal of Cell Biology, 1981