A Sequential Strand-Displacement Strategy Enables Efficient Six-Step DNA-Templated Synthesis
Authors:
Open Access
- 14 June 2011
- journal article
- research article
- Published by American Chemical Society (ACS) in Journal of the American Chemical Society
- Vol. 133 (26) , 9972-9975
Abstract
We developed a sequential strand-displacement strategy for multistep DNA-templated synthesis (DTS) and used it to mediate an efficient six-step DTS that proceeded in 35% overall yield (83% average yield per step). The efficiency of this approach and the fact that the final product remains linked to a DNA sequence that fully encodes its reaction history suggests its utility for the translation of DNA sequences into high-complexity synthetic libraries suitable for in vitro selection.Keywords
Funding Information
- National Institute of General Medical Sciences
- Howard Hughes Medical Institute
This publication has 39 references indexed in Scilit:
- In Vitro Selection of a DNA-Templated Small-Molecule Library Reveals a Class of Macrocyclic Kinase InhibitorsJournal of the American Chemical Society, 2010
- DNA as a Versatile Chemical Component for Catalysis, Encoding, and StereocontrolAngewandte Chemie International Edition, 2010
- An in vitro translation, selection and amplification system for peptide nucleic acidsNature Chemical Biology, 2009
- A Yoctoliter-Scale DNA Reactor for Small-Molecule EvolutionJournal of the American Chemical Society, 2009
- DNA-Templated Polymerization of Side-Chain-Functionalized Peptide Nucleic Acid AldehydesJournal of the American Chemical Society, 2008
- DNA‐Templated Organic Synthesis: Nature's Strategy for Controlling Chemical Reactivity Applied to Synthetic MoleculesAngewandte Chemie International Edition, 2004
- DNA-Templated Organic Synthesis and Selection of a Library of MacrocyclesScience, 2004
- Efficient and Sequence-Specific DNA-Templated Polymerization of Peptide Nucleic Acid AldehydesJournal of the American Chemical Society, 2003
- Expanding the Reaction Scope of DNA-Templated SynthesisAngewandte Chemie International Edition, 2002
- The Generality of DNA-Templated Synthesis as a Basis for Evolving Non-Natural Small MoleculesJournal of the American Chemical Society, 2001