Gallic Acid Induces Neuronal Cell Death through Activation of c‐Jun N‐Terminal Kinase and Downregulation of Bcl‐2

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Abstract
Oxidative stress induced by reactive oxygen species (ROS) is strongly associated with the pathogenesis of various neurodegenerative disorders, including Alzheimer's disease. We investigated the possible combined effects of gallic acid and resveratrol, which are major antioxidants present in fruit, including grapes, on PC12 rat pheochromocytoma (PC12) cell death. Gallic acid did not protect against H2O2‐induced PC12 cell death; it reduced the viability of PC12 cells in a dose‐dependent manner. Gallic acid also induced cleavage of poly (ADP‐ribose) polymerase, which is strongly related to apoptosis in neurons. Gallic acid induced the phosphorylation of c‐Jun N‐terminal protein kinase (JNK) and the downregulation of Bcl‐2 in PC12 cells. Treatment of PC12 cells with resveratrol increased their viability in a dose‐dependent manner by blocking the activation of JNK and the downregulation of Bcl‐2. Furthermore, gallic acid led to a progressive reduction in the viability of vector‐transfected PC12 cells, which was delayed in PC12 cells that overexpressed Bcl‐2. The JNK inhibitor SP600125 protected against gallic acid‐induced PC12 cell death. Collectively, these findings suggest that the combined effects of dietary phenolic phytochemicals on oxidative neuronal cell death and antioxidants differ in ROS‐mediated neuronal cell death.

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