Sex determination of the Pampas deer (Ozotoceros bezoarticus) via high-resolution melting analysis
Preprint
- 31 March 2025
- preprint
- Published by Springer Nature in Research Square
Abstract
We present a fast and reliable genetic method for sexing Pampas deer (Ozotoceros bezoarticus) via high-resolution melting (HRM) analysis of amplicons obtained through real-time polymerase chain reaction (PCR-RT). This method employs the KY1/KY2 primer set to amplify an informative fragment of the Amelogenin gene that accurately determines the sex of Pampas deer. This approach is effective for analyzing noninvasive samples with low DNA quality and quantity. Additionally, this protocol can be easily adapted for sexing noninvasive samples from other deer and ungulate species.Keywords
All Related Versions
- Version 1, 2025-03-31, Research Square
- Published version: Mammal Research, 1.
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