Apoplastic barriers are essential for nodule formation and nitrogen fixation in Lotus japonicus
Authors:
- Defeng ShenDSDefeng Shen
- Nikola MicicNMNikola Micic
- Rafael E. VenadoRVRafael E. Venado
- Nanna BjarnholtNBNanna Bjarnholt
- Christoph CrocollCCChristoph Crocoll
- Daniel Pergament PerssonDPDaniel Pergament Persson
- Sebastian SamwaldSSSebastian Samwald
- Stanislav KoprivaSKStanislav Kopriva
- Philip WesthoffPWPhilip Westhoff
- Sabine MetzgerSMSabine Metzger
- Ulla NeumannUNUlla Neumann
- Ryohei Thomas NakanoRNRyohei Thomas Nakano
- 21 March 2025
- journal article
- Published by American Association for the Advancement of Science (AAAS) in Science
- Vol. 387 (6740) , 1281-1286
Abstract
Establishment of the apoplastic root barrier known as the Casparian strip occurs early in root development. In legumes, this area overlaps with nitrogen-fixing nodule formation, which raises the possibility that nodulation and barrier formation are connected. Nodules also contain Casparian strips, yet, in this case, their role is unknown. We established mutants with defective barriers in Lotus japonicus . This revealed that effective apoplastic blockage in the endodermis is important for root-to-shoot signals underlying nodulation. Our findings further revealed that in nodules, the genetic machinery for Casparian strip formation is shared with roots. Apoplastic blockage controls the metabolic source-sink status required for nitrogen fixation. This identifies Casparian strips as a model system to study spatially constrained symbiotic plant-microbe relationships.Keywords
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