A Direct Radioimmunoassay of Corticosterone in Rat Serum

Authors:
  • CGCelso Gomez-Sanchez
    Celso Gomez-Sanchez
  • BMBarbara A. Murry
    Barbara A. Murry
  • DKDavid C. Kem
    David C. Kem
  • NKNorman M. Kaplan
    Norman M. Kaplan
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Abstract
A direct radioimmunoassay of corticosterone is described requiring as little as 1-2 mul of rat serum. After methanol inactivation of CBG, serum was assayed with a relatively specific antibody raised against corticosterone-21-hemisuccinate. While this antibody has a significant cross-reaction with cortisol, prior purification is not necessary since cortisol is not produced in the rat.