Precise fine-turning of GhTFL1 by base editing tools defines ideal cotton plant architecture

Authors:
Open Access
Publisher website
Google Scholar
Abstract
Background: CRISPR/Cas-derived base editor enables precise editing of target sites and has been widely used for basic research and crop genetic improvement. However, the editing efficiency of base editors at different targets varies greatly. Results: Here, we develop a set of highly efficient base editors in cotton plants. GhABE8e, which is fused to conventional nCas9, exhibits 99.9% editing efficiency, compared to GhABE7.10 with 64.9%, and no off-target editing is detected. We further replace nCas9 with dCpf1, which recognizes TTTV PAM sequences, to broaden the range of the target site. To explore the functional divergence of TERMINAL FLOWER 1 (TFL1), we edit the non-coding and coding regions of GhTFL1 with 26 targets to generate a comprehensive allelic population including 300 independent lines in cotton. This allows hidden pleiotropic roles for GhTFL1 to be revealed and allows us to rapidly achieve directed domestication of cotton and create ideotype germplasm with moderate height, shortened fruiting branches, compact plant, and early-flowering. Further, by exploring the molecular mechanism of the GhTFL1L86P and GhTFL1K53G+S78G mutations, we find that the GhTFL1L86P mutation weakens the binding strength of the GhTFL1 to other proteins but does not lead to a complete loss of GhTFL1 function. Conclusions: This strategy provides an important technical platform and genetic information for the study and creation of ideal plant architecture.
Funding Information
  • Science and Technology Innovation 2030 (2022ZD0402001-04)
  • Ministry of Agriculture and Rural Affairs (2023ZD04039-01)
  • National Natural Science Fund of China for Distinguished Young Scholars (32325039)
  • The National Natural Science Foundation of China (32272128)
  • STI 2030-Major Projects (2023ZD04074)
  • China Postdoctoral Science Foundation (2023M741299)

This publication has 70 references indexed in Scilit: