Femtosecond photonic viral inactivation probed using solid-state nanopores

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Abstract
We report on detection of virus inactivation using femtosecond laser radiation by measuring the conductance of a solid state nanopore designed for detecting single particles. Conventional methods of assaying for viral inactivation based on plaque forming assays require 24–48 h for bacterial growth. Nanopore conductance measurements provide information on morphological changes at a single virion level. We show that analysis of a time series of nanopore conductance can quantify the detection of inactivation, requiring only a few minutes from collection to analysis. Morphological changes were verified by dynamic light scattering. Statistical analysis maximizing the information entropy provides a measure of the log reduction value. This work provides a rapid method for assaying viral inactivation with femtosecond lasers using solid-state nanopores.

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