THE EFFECTS OF RED BLOOD CELL EXTRACTS ON THE PROLIFERATION OF ERYTHROCYTE PRECURSOR CELLS, IN VIVO

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Abstract
Saline incubation extracts of mature erythrocytes were assayed in vivo by a variety of techniques in order to study their ability to modify the proliferation of maturing erythroid cells. Using comparable extracts from granulocytes and lymphocytes, the specificity of the effect of the red cell extract for erythroid cells was confirmed by measurement of autoradiographic labeling indices, radio-Fe incorporation and spleen colony growth. The erythroid cells were very sensitive to the effects of the extract, as little as 10 .mu.g/mouse producing a maximum effect on Fe incorporation. The extract apparently does not block erythroid cell proliferation completely but simply lengthens the cell cycle, mainly by increasing the G1 phase of the cycle. There was no effect on the committed erythroid precursor cells. The in vivo activity, specificity and non-toxicity to the cells, together with the cells'' sensitivity to red cell extract suggest that this inhibitor may play a physiological role in the control of red cell production.

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