The structural characterization of endogenous factors from Vinca rosea crown gall tumors that promote cell division of tobacco cells.
Authors:
- D G LynnDLD G Lynn
- R H ChenRCR H Chen
- K S ManningKMK S Manning
- H N WoodHWH N Wood
- 1 February 1987
- journal article
- research article
- Published by National Academy of Sciences in Proceedings of the National Academy of Sciences
- Vol. 84 (3) , 615-619
Abstract
The ability of two compounds, a cytokinin and an auxin, to stimulate tobacco cell growth and differentiation has been known for > 30 years, but the molecular mechanism of this activation is still unknown. Previous reports of factors endogenous in crown gall tumors of Vinca rosea that could replace the cytokinin requirement in tobacco cell culture has motivated an investigation of these tissues. The optimization of a reverse-phase isolation scheme has led to the purification of sufficient material to allow for the identification of six different related compounds. The structures of two of these compounds have been assigned as a set of epimeric dehydrodiconiferyl alcohol .beta.-D-glycosides. The structure of these compounds suggests that they would most likely be derived from the plant cell wall.Keywords
This publication has 11 references indexed in Scilit:
- Two-Dimensional Nuclear Magnetic Resonance SpectroscopyScience, 1986
- Molecular Basis for the Auxin-Independent Phenotype of Crown Gall Tumor TissuesScience, 1986
- A plant cell factor induces Agrobacterium tumefaciens vir gene expressionProceedings of the National Academy of Sciences, 1986
- T-DNA of Agrobacterium tumefaciens encodes an enzyme of cytokinin biosynthesis.Proceedings of the National Academy of Sciences, 1984
- STRUCTURE AND FUNCTION OF THE PRIMARY CELL WALLS OF PLANTSAnnual Review of Biochemistry, 1984
- Stable incorporation of plasmid DNA into higher plant cells: the molecular basis of crown gall tumorigenesisCell, 1977
- Glucosyl Zeatin and Glucosyl Ribosylzeatin from Vinca rosea L. Crown Gall Tumor TissuePlant Physiology, 1977
- Ribosyl- trans -Zeatin, A Major Cytokinin Produced by Crown Gall Tumor TissueProceedings of the National Academy of Sciences, 1974
- STUDIES ON THE DISTRIBUTION AND PROPERTIES OF A NEW CLASS OF CELL DIVISION-PROMOTING SUBSTANCES FROM HIGHER PLANT SPECIESProceedings of the National Academy of Sciences, 1969
- Organic Growth Factor Requirements of Tobacco Tissue CulturesPhysiologia Plantarum, 1965