A Scaleable and Defined System for Generating Neural Stem Cells from Human Embryonic Stem Cells
Open Access
- 9 November 2006
- journal article
- research article
- Published by Oxford University Press (OUP) in Stem Cells
- Vol. 25 (3) , 731-737
Abstract
The ability to differentiate human ESCs (hESCs) to defined lineages in a totally controlled manner is fundamental to developing cell-based therapies and studying human developmental mechanisms. We report a novel, scaleable, and widely applicable system for deriving and propagating neural stem cells from hESCs without the use of animal products, proprietary formulations, or genetic manipulation. This system provides a definitive platform for studying human neural development and has potential therapeutic implications.Keywords
This publication has 66 references indexed in Scilit:
- Long-term self-renewal and directed differentiation of human embryonic stem cells in chemically defined conditionsProceedings of the National Academy of Sciences, 2006
- Defined culture conditions of human embryonic stem cellsProceedings of the National Academy of Sciences, 2006
- Neurotrophins mediate human embryonic stem cell survivalNature Biotechnology, 2006
- Derivation of human embryonic stem cells in defined conditionsNature Biotechnology, 2006
- Regenerative medicine in Parkinson's disease: generation of mesencephalic dopaminergic cells from embryonic stem cellsCurrent Opinion in Biotechnology, 2005
- Neural stem cells as a potential source of oligodendrocytes for myelin repairJournal of the Neurological Sciences, 2005
- Human embryonic stem cells derived without feeder cellsThe Lancet, 2005
- Neural Stem Cell Therapy in the Aging Brain: Pitfalls and PossibilitiesJournal of Hematotherapy & Stem Cell Research, 2003
- Embryonic Stem Cells Express Neuronal Properties in VitroDevelopmental Biology, 1995
- Evidence for Involvement of Activin A and Bone Morphogenetic Protein 4 in Mammalian Mesoderm and Hematopoietic DevelopmentMolecular and Cellular Biology, 1995