Western Blot Techniques
Author:
- 14 May 2017
- book chapter
- Published by Springer Nature
- Vol. 1606, 133-139
Abstract
The Western blot is an important laboratory technique that allows for specific identification and characterization of proteins. Sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE)-separated proteins are electophoretically transferred to a polyvinylidene fluoride (PVDF) membrane which is then incubated with specific antibodies, then developed to show the protein of interest. Here, we describe the transfer and detection of Outer surface protein A (OspA), a protein only found on the surface of Borrelia burgdorferi, the bacteria responsible for Lyme disease.Keywords
This publication has 5 references indexed in Scilit:
- Application of Nanotrap technology for high sensitivity measurement of urinary outer surface protein A carboxyl-terminus domain in early stage Lyme borreliosisJournal of Translational Medicine, 2015
- The necessity of and strategies for improving confidence in the accuracy of western blotsExpert Review of Proteomics, 2014
- Epidemiology of Lyme Borreliosis2009
- Quantitative femto- to attomole immunodetection of regulated secretory vesicle proteins critical to exocytosisAnalytical Biochemistry, 2002
- Early and late antibody responses to full-length and truncated constructs of outer surface protein A of Borrelia burgdorferi in Lyme diseaseInfection and Immunity, 1995