Single nucleotide polymorphism genotyping of bovine milk protein genes using the tetra‐primer ARMS‐PCR
Authors:
- 17 September 2003
- journal article
- research article
- Published by Wiley in Journal of Animal Breeding and Genetics
- Vol. 120 (5) , 331-337
Abstract
Summary: We demonstrate the use and optimization of the tetra‐primer amplification refractory mutation system‐polymerase chain reaction (tetra‐primer ARMS‐PCR) as an efficient technique to genotype single nucleotide polymorphism (SNP) of milk protein genes. The technique utilizes a set of four primers in a single PCR reaction tube without the need of post‐PCR manipulations to accomplish an efficient classification. The technique was successfully applied to genotype SNP in the bovine κ‐casein, β‐casein, αS1‐casein and α‐lactalbumin genes.Keywords
This publication has 13 references indexed in Scilit:
- Detection of the G→T Polymorphism at the Sp1 Binding Site of the Collagen Type I α 1 Gene by a Novel Arms-PCR MethodGenetic Testing, 2002
- An efficient procedure for genotyping single nucleotide polymorphismsNucleic Acids Research, 2001
- Overlapping PCR for Bidirectional PCR Amplification of Specific Alleles: A Rapid One-Tube Method for Simultaneously Differentiating Homozygotes and HeterozygotesGenome Research, 1997
- Single-Base Polymorphisms Within the 5’ Flanking Region of the Bovine α-Lactalbumin GeneJournal of Dairy Science, 1997
- Genotyping bovine milk proteins using allele discrimination by primer length and automated DNA sizing technologyAnimal Genetics, 1995
- Direct sequencing and bidirectional allelle specific polymerase chain reaction of the bovine β‐casein B variantAnimal Genetics, 1992
- Detection of bovine αs1‐casein genomic variants using the allele‐specific polymerase chain reactionAnimal Genetics, 1992
- Detection of multiple β‐casein (CASB) alleles by amplification created restriction sites (ACRS)Animal Genetics, 1992
- Genotyping of Bovine Kappa-Casein Loci Following DNA Sequence AmplificationNature Biotechnology, 1990
- Polymerase chain reaction amplification of bovine β‐lactoglobulin genomic sequences and identification of genetic variants by RFLP analysisAnimal Biotechnology, 1990