Phenotypic Characterization of CD7 +, CD3 +, and CD8+ Lymphocytes From First Trimester Human Decidua Using Two‐Color Flow Cytometry

Authors:
Publisher website
Google Scholar
Abstract
PROBLEM: There is increasing evidence that decidual lymphocytes play a major role in local interactions at the fetomaternal interface.METHOD: In this paper we use two‐color flow cytometry to delineate the phenotype of lymphocytes obtained from human early decidua by mechanical dispersal technique.RESULTS: The most abundant decidual lymphocytes expressed CD7, CD38, CD56, and CD2 markers, relatively small proportions of CD3 +, CD8 +, CD4 +, CD16 +, CD45RA+, CD11b+, and Leu8+ cells were also present. The vast majority of decidual CD7+ lymphocytes expressed CD38, CD2, and CD56 markers and were CD3 −, CD8 −, CD 16 −, and CD57 −. Decidual CD3+ lymphocytes were weakly staining for TCR α/β, lacked T‐cell receptor (TCR) γ/δ molecules, and ∼40% of them expressed HLA‐DR. All decidual CD8+ lymphocytes were CD2+ and the majority of them expressed CD38, CD56, and CD7 markers and were CD3−at the same time CD8 + CD7− lymphocytes were found in decidua. According to the expression of the CD45RA marker, decidual CD8 + lymphocytes could be divided into two subsets: CD8 + CD45RA + CD56 + and CD8 + CD45RA −.CONCLUSIONS: These data clearly demonstrate that decidual lymphocytes display phenotypical features different from those of their counterparts in peripheral blood.

This publication has 33 references indexed in Scilit: