Aβ production as consequence of cellular death of a human neuroblastoma overexpressing APP
Authors:
- Elena SerranoESElena Serrano
- Marı́a J BullidoMBMarı́a J Bullido
- Fernando ValdiviesoFVFernando Valdivieso
- 26 June 2004
- journal article
- Published by Wiley in FEBS Letters
- Vol. 570 (1-3) , 114-118
Abstract
In human brain the Aβ peptide is produced mainly by neurons and the overexpression of amyloid precursor protein (APP) that involves an increase in Aβ secretion, has been observed in some areas of the Alzheimer's disease patients brain. We have generated two stably transfected human neuroblastoma lines which overexpress APP; both of them secreted Aβ and showed morphological changes and cell death with apoptotic program characteristics. Interestingly, coculture experiments with the untransfected human neuroblastoma cell line showed that the Aβ peptide was not responsible for the death in those cell lines; additionally, we indicate that upon cell death, Aβ peptide is secreted into cell medium.Keywords
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