Cascade Dams and Seasonality Jointly Structure Gut Microbiome Biogeography in Saurogobio punctatus

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Abstract
Cascade dams fragment river habitats, but how seasonal hydrology modulates the biogeography and assembly of fish gut microbiota remains unclear. We surveyed gut bacterial communities of the omnivorous fish Saurogobio punctatus across 10 reaches separated by cascade dams in the Qijiang River during the wet (summer) and dry (winter) seasons using 16S rRNA gene amplicon sequencing. Sampling was synchronized among reaches to minimize temporal variability. Winter exhibited stronger differentiation among reaches and a steeper distance–decay pattern, and reach-scale environmental heterogeneity (especially dissolved inorganic nitrogen) was more stable under weak hydrodynamics. Null model analyses showed that stochastic processes dominated in summer, with dispersal-related processes and drift being prominent under high connectivity, whereas deterministic assembly increased in winter and was mainly associated with homogeneous selection. Compositionality-aware differential abundance analysis (ANCOM-BC2) identified 409 genera with a significant seasonal differential abundance after adjusting for reach (FDR q < 0.05). Random forest classification, used as a complementary prediction-oriented feature-ranking analysis, indicated higher reach discriminability in winter, with Nitrospirota ranking among the top features. PLS-PM indicated that α-diversity had the strongest direct association with β-diversity in the specified model, whereas spatial and environmental effects were linked to β-diversity mainly through indirect, α-diversity-mediated pathways. Biologically, α-diversity may reflect an integrative summary of the within-gut taxon pool shaped by host filtering and environmentally derived inputs (e.g., diet- and habitat-associated sources), which can influence the magnitude of between-reach compositional turnover. Together, these results show that seasonal hydrological regimes tune spatial turnover and assembly of fish gut microbiota in cascade-regulated rivers.
Funding Information
  • National Key Research and Development Program of China (2022YFC3204201)

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