Spectral Imaging Analysis for Ultrasensitive Biomolecular Detection Using Gold-Capped Nanowire Arrays
Open Access
Abstract
A spectral integration combined with a threshold method for the analysis of spectral scanning surface plasmon resonance (SPR) images can significantly increase signal recognition at low concentration of antibody solution. The 12-well SPR sensing plates consisted of gold-capped nanowire arrays with 500-nm period, 80-nm linewidth and 50-nm gold thickness which were used for generating multiple SPR images. A threshold method is introduced to eliminate background noises in spectral scanning images. Combining spectral integration and the threshold method, the detection limit of antibody concentration was 1.23 ng/mL. Using multiple-well SPR sensing plates and the proposed analytical method, multiple kinetic responses with spectral and spatial information on different sensing areas can be sensitively measured.Keywords
This publication has 31 references indexed in Scilit:
- Wavelength-Scanning SPR Imaging Sensors Based on an Acousto-Optic Tunable Filter and a White Light LaserSensors, 2017
- High-throughput imaging surface plasmon resonance biosensing based on an adaptive spectral-dip tracking schemeOptics Express, 2016
- Fabrication of DNA Microarrays on Polydopamine-Modified Gold Thin Films for SPR Imaging MeasurementsLangmuir, 2013
- Real-time full-spectral imaging and affinity measurements from 50 microfluidic channels using nanohole surface plasmon resonanceLab on a Chip, 2012
- Parallel-scan based microarray imager capable of simultaneous surface plasmon resonance and hyperspectral fluorescence imagingBiosensors and Bioelectronics, 2011
- Real-time protein biosensor arrays based on surface plasmon resonance differential phase imagingBiosensors and Bioelectronics, 2008
- A Multistep Chemical Modification Procedure To Create DNA Arrays on Gold Surfaces for the Study of Protein−DNA Interactions with Surface Plasmon Resonance ImagingJournal of the American Chemical Society, 1999
- Near-Infrared Surface Plasmon Resonance Measurements of Ultrathin Films. 1. Angle Shift and SPR Imaging ExperimentsAnalytical Chemistry, 1999
- Surface Plasmon Resonance Imaging Measurements of DNA Hybridization Adsorption and Streptavidin/DNA Multilayer Formation at Chemically Modified Gold SurfacesAnalytical Chemistry, 1997
- High sensitivity surface plasmon resonace sensor based on phase detectionSensors and Actuators B: Chemical, 1996