Production of Basta-resistant transgenic Dendrobium linguella orchids carrying the cytochrome P450scc CYP11A1 gene

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Abstract
Dendrobium linguella orchids were genetically transformed with a construct containing the bar gene and the cytochrome P450scc cyp11A1 gene to obtain plants with improved productivity and herbicide resistance. The bar gene served as a bialaphos resistance marker. Following 8–10 months of selection, 17 transformed plants were obtained and analyzed molecularly. PCR using two primer pairs for each gene confirmed integration of bar and cyp11A1 into the genome of all regenerated clones. The study reports production of Dendrobium linguella plants carrying both transgenes. Editorial note (2026): This abstract was prepared for reference purposes using artificial intelligence technology, based on the original publication, without the involvement of the author(s).