The main aim of this study was to determine the chemical composition of the essential oil from the leaves of Callistemon citrinus grown in the South-West of Iran. The essential oil was extracted by hydrodistillation and analyzed by GC-MS and GC-FID. Thirty-six compounds were identified and the major constituents were 1,8-cineole (34.2 %), α-pinene (29.0 %), α-terpineol (10.7 %), α-phellandrene (9.0 %) and limonene (3.4 %).