Neural stem and progenitor cells shorten S-phase on commitment to neuron production
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Open Access
- 11 January 2011
- journal article
- Published by Springer Nature in Nature Communications
- Vol. 2 (1) , 154
Abstract
During mammalian cerebral cortex development, the G1-phase of the cell cycle is known to lengthen, but it has been unclear which neural stem and progenitor cells are affected. In this paper, we develop a novel approach to determine cell-cycle parameters in specific classes of neural stem and progenitor cells, identified by molecular markers rather than location. We found that G1 lengthening was associated with the transition from stem cell-like apical progenitors to fate-restricted basal (intermediate) progenitors. Unexpectedly, expanding apical and basal progenitors exhibit a substantially longer S-phase than apical and basal progenitors committed to neuron production. Comparative genome-wide gene expression analysis of expanding versus committed progenitor cells revealed changes in key factors of cell-cycle regulation, DNA replication and repair and chromatin remodelling. Our findings suggest that expanding neural stem and progenitor cells invest more time during S-phase into quality control of replicated DNA than those committed to neuron production.Keywords
This publication has 58 references indexed in Scilit:
- Forced G1-phase reduction alters mode of division, neuron number, and laminar phenotype in the cerebral cortexProceedings of the National Academy of Sciences, 2009
- The Glial Nature of Embryonic and Adult Neural Stem CellsAnnual Review of Neuroscience, 2009
- Intermediate Neuronal Progenitors (Basal Progenitors) Produce Pyramidal–Projection Neurons for All Layers of Cerebral CortexCerebral Cortex, 2009
- Tbr2 Directs Conversion of Radial Glia into Basal Precursors and Guides Neuronal Amplification by Indirect Neurogenesis in the Developing NeocortexNeuron, 2008
- The T-box transcription factor Eomes/Tbr2 regulates neurogenesis in the cortical subventricular zoneGenes & Development, 2008
- Distinct behaviors of neural stem and progenitor cells underlie cortical neurogenesisJournal of Comparative Neurology, 2008
- Fanconi DNA repair pathway is required for survival and long‐term maintenance of neural progenitorsThe EMBO Journal, 2008
- Conditional inactivation of Fbxw7 impairs cell-cycle exit during T cell differentiation and results in lymphomatogenesisJournal of Experimental Medicine, 2007
- Selective utilization of nonhomologous end-joining and homologous recombination DNA repair pathways during nervous system developmentProceedings of the National Academy of Sciences, 2006
- Cdc25A phosphatase: combinatorial phosphorylation, ubiquitylation and proteolysisOncogene, 2004