Simplified Approach to HPLC Precolumn Fluorescent Labeling of Carbohydrates: N-(2-Pyridinyl)-glycosylamnes
Authors:
- 1 March 1987
- journal article
- research article
- Published by Taylor & Francis in Journal of Carbohydrate Chemistry
- Vol. 6 (1) , 129-139
Abstract
Reducing sugars were fluorescently labeled by condensation with 2-aminopyridine. The reaction was quantitative and the product was sufficiently stable so that a subsequent reduction step (as in reductive amination) proved unnecessary. The products have been characterized as N-(2-pyridinyl)-glycosylamines. Oligosaccharides labeled in this manner exhibit important analytical characteristics. They: a) are stable under both HPLC separation and prolonged storage; b) have improved chromatographic resolution; c) regenerate original material by weak acid hydrolysis.Keywords
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