TaqMan-Based Real-Time PCR Method for Detection of Yersinia pseudotuberculosis in Food
- 15 October 2008
- journal article
- research article
- Published by American Society for Microbiology in Applied and Environmental Microbiology
- Vol. 74 (20) , 6465-6469
Abstract
A sensitive and specific assay for detection of food-borne pathogenic Yersinia pseudotuberculosis was developed. The primer-probe set was designed to target a 157-bp sequence of the chromosomally located gene ail . The complete method, including an internal amplification control, was evaluated for several different food items.Keywords
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