A femtosecond Raman generator for long wavelength two-photon and third harmonic generation imaging
Open Access
- 27 September 2016
- journal article
- conference paper
- Published by AIP Publishing in APL Photonics
- Vol. 1 (9) , 091303
Abstract
We demonstrate a femtosecond single pass Raman generator based on an YVO4 crystal pumped by a high energy fiber laser at a wavelength of 1064 nm and a repetition rate of 1 MHz. The Raman generator shifts the pump wavelength to 1175 nm, in a broadband spectrum, making it suitable for multi-photon microscopy. We use the Raman generator for third harmonic generation imaging of live plant specimens as well as for two-photon fluorescence imaging of red fluorescent protein expressing HeLa cells. We demonstrate that the photo-damage to a live specimen is low.Keywords
Funding Information
- Medical Research Council (MR/K015583/1)
- Medical Research Scotland (Vac- 802-2015)
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