Ethanol stimulates epithelial sodium channels by elevating reactive oxygen species

Authors:
  • AGAgustin Gonzalez-Vicente
    Agustin Gonzalez-Vicente
  • NHNancy Hong
    Nancy Hong
  • JGJeffrey L. Garvin
    Jeffrey L. Garvin
  • ASAndreas Schwingshackl
    Andreas Schwingshackl
  • AEAmity F. Eaton
    Amity F. Eaton
  • QYQiang Yue
    Qiang Yue
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Abstract
Alcohol affects total body sodium balance, but the molecular mechanism of its effect remains unclear. We used single-channel methods to examine how ethanol affects epithelial sodium channels (ENaC) in A6 distal nephron cells. The data showed that ethanol significantly increased both ENaC open probability ( Po) and the number of active ENaC in patches ( N). 1-Propanol and 1-butanol also increased ENaC activity, but iso-alcohols did not. The effects of ethanol were mimicked by acetaldehyde, the first metabolic product of ethanol, but not by acetone, the metabolic product of 2-propanol. Besides increasing open probability and apparent density of active channels, confocal microscopy and surface biotinylation showed that ethanol significantly increased α-ENaC protein in the apical membrane. The effects of ethanol on ENaC Poand N were abolished by a superoxide scavenger, 4-hydroxy-2,2,6,6-tetramethylpiperidinyloxy (TEMPOL) and blocked by the phosphatidylinositol 3-kinase inhibitor LY294002. Consistent with an effect of ethanol-induced reactive oxygen species (ROS) on ENaC, primary alcohols and acetaldehyde elevated intracellular ROS, but secondary alcohols did not. Taken together with our previous finding that ROS stimulate ENaC, the current results suggest that ethanol stimulates ENaC by elevating intracellular ROS probably via its metabolic product acetaldehyde.

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