Lactoferrin-EGCG covalent complex promotes osteoblast differentiation via TGF-β/BMP signaling pathway
Authors:
- FengJiao FANFFFengJiao FAN
- XiaoNi LIANXLXiaoNi LIAN
- ZhenNi ZHAIZZZhenNi ZHAI
- HongKai XIEHXHongKai XIE
- HuiLing CHENHCHuiLing CHEN
- JiaJie YINJYJiaJie YIN
- XiaoYi JIANGXJXiaoYi JIANG
- Jian DingJDJian Ding
- Yong FangYFYong Fang
Open Access
- 23 January 2026
- journal article
- Published by Science China Press., Co. Ltd. in SCIENTIA SINICA Vitae
- Vol. 56 (7) , 1748-1761
Abstract
乳铁蛋白和表没食子儿茶素没食子酸酯具有良好的促成骨活性, 然而, 二者形成的复合物其促成骨活性及其机制尚不明确. 本研究以共价作用0.5 h的LF-EGCG复合物为研究对象, MC3T3-E1成骨细胞为研究模型, 深入探究了LF-EGCG复合物的促成骨机制. 结果表明, LF-EGCG复合物能够显著促进MC3T3-E1细胞增殖分化, 100 μg/mL的LF-EGCG复合物显著增加了碱性磷酸酶活性, 使其相比LF提高1.65倍. LF-EGCG复合物显著促进了MC3T3-E1细胞的矿化并上调Runx2、OSX、OPN的基因表达水平. 与此同时, LF-EGCG复合物增加了S期细胞的比例, 并且在一定程度上逆转了高浓度EGCG对MC3T3-E1细胞迁移能力的抑制作用. 此外, LF-EGCG复合物能够通过调节RANKL和OPG的分泌, 降低RANKL/OPG比例. 最后, 转录组测序与RT-qPCR结果表明, LF-EGCG复合物通过调控TGF-β/BMP通路发挥促成骨活性. 本研究为LF-EGCG共价复合物在功能食品领域的研发与应用奠定了理论基础.Keywords
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