MISHANDLING OF COPPER BY ALBUMIN: ROLE IN REDOX-CYCLING AND OXIDATIVE STRESS IN PREECLAMPSIA PLASMA
Authors:
- Valerian E. KaganVKValerian E. Kagan
- Vladimir A. TyurinVTVladimir A. Tyurin
- Grigory G. BorisenkoGBGrigory G. Borisenko
- James P. FabisiakJFJames P. Fabisiak
- Carl A. HubelCHCarl A. Hubel
- Roberta B. NessRNRoberta B. Ness
- Robin GandleyRGRobin Gandley
- Margaret K. McLaughlinMMMargaret K. McLaughlin
- James M. RobertsJRJames M. Roberts
- 1 January 2001
- journal article
- Published by Taylor & Francis in Hypertension in Pregnancy
- Vol. 20 (3) , 221-241
Abstract
Objective. To test the hypothesis that enhanced oxidative stress during pregnancies complicated by preeclampsia is associated with improper copper (Cu) binding by plasma albumin, resulting in enhanced Cu redox-cycling activity and that altered Cu binding, in turn, is caused by interactions of excessive amounts of free fatty acids with albumin.Study Design. We studied binding and redox-cycling activity of Cu in 17 normal pregnancy and 17 preeclampsia plasma samples. Binding of exogenous Cu in plasma samples was quantified indirectly using spectrophotometric measurements of its complex with a specific chelator of Cu(I), bathocuproine disulfonate. Redox-cycling activity of Cu in plasma samples was estimated by electron paramagnetic resonance (EPR) spectroscopy of ascorbate radicals formed during one-electron oxidation of ascorbate by redox-active catalytic Cu. Formation of Cu/albumin complexes in model systems in the presence and absence of fatty acids was studied using EPR spectroscopy of Cu(II)/albumin.Res...Keywords
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