Role for BLM in replication-fork restart and suppression of origin firing after replicative stress
Authors:
- 24 June 2007
- journal article
- research article
- Published by Springer Nature in Nature Structural & Molecular Biology
- Vol. 14 (7) , 677-679
Abstract
Mutations in BLM give rise to Bloom's syndrome, a genetic disorder associated with cancer predisposition and chromosomal instability. Using a dual-labeling system in isolated chromosome fibers, we show that the BLM protein is required for two aspects of the cellular response to replicative stress: efficient replication-fork restart and suppression of new origin firing. These functions require the helicase activity of BLM and the Thr99 residue targeted by stress-activated kinases.Keywords
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