Evaluation of Direct Ligand-Receptor Interactions by Photoaffinity Labeling
- 30 November 2023
- book chapter
- Published by Springer Nature
- Vol. 2731, 231-240
Abstract
Binding assays provide ultimate proof that a particular peptide and receptor kinase (RK) do indeed function as a ligand-receptor pair. Among available binding assays, proximity-induced photoaffinity labeling is superior for confirming direct contact between the peptide ligand and the receptor. Our binding assay employs covalent photoaffinity labeling followed by immunoprecipitation to specifically evaluate the ligand binding activity of the target RKs. Here, we describe a protocol for the synthesis of photoactivatable peptide ligands and the UV-induced formation of covalent bonds between photoaffinity ligands and RKs. Key words Photoaffinity labeling Receptor kinase Peptide ligand Ligand-receptor interactionKeywords
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