Comparison of ITS and IGS1 regions for strain typing of clinical and non-clinical isolates of Pichia anomala
- 1 February 2004
- journal article
- research article
- Published by Microbiology Society in Journal of Medical Microbiology
- Vol. 53 (2) , 119-123
Abstract
Pichia anomalais an emerging nosocomial pathogen and there is a need for methods that distinguish between differentP. anomalastrains. In the typing of several clinical as well as non-clinicalP. anomalastrains, the sequence variation of the internal transcribed spacer (ITS) was found to be inadequate for typing purposes. The intergenic spacer 1 (IGS1) region of the rDNA of severalP. anomalastrains was therefore investigated in detail. The IGS1 region (which varied from 1213 to 1231 bp in length) was interspersed with repeats and had more variation than the ITS regions. Comparative analysis in cases where analysis by the ITS was ambiguous clearly revealed the IGS1 region to be a more discriminatory tool in the typing ofP. anomalastrains.Keywords
This publication has 21 references indexed in Scilit:
- Multi-locus sequence typing: a tool for global epidemiologyTrends in Microbiology, 2003
- CLOURE: Clustal Output Reformatter, a program for reformatting ClustalX/ClustalW outputs for SNP analysis and molecular systematicsNucleic Acids Research, 2003
- Sequence Analysis of the Ribosomal DNA Intergenic Spacer 1 Regions of Trichosporon SpeciesJournal of Clinical Microbiology, 2002
- Outbreak of Pichia anomala Infection in the Pediatric Service of a Tertiary-Care Center in Northern IndiaJournal of Clinical Microbiology, 2001
- Gapped BLAST and PSI-BLAST: a new generation of protein database search programsNucleic Acids Research, 1997
- The 5S rRNA and the rRNA intergenic spacer of the two varieties ofCryptococcus neoformansMedical Mycology, 1995
- CLUSTAL W: improving the sensitivity of progressive multiple sequence alignment through sequence weighting, position-specific gap penalties and weight matrix choiceNucleic Acids Research, 1994
- Primary structure of the non-transcribed spacer region and flanking sequences of the ribosomal DNA of Neurospora crassa and comparison with other organismsBiochemical and Biophysical Research Communications, 1990
- Hansenula anomala: A New Fungal PathogenArchives of internal medicine (1960), 1988
- Infective endocarditis of a bicuspid aortic valve caused by hansenula anomalaThe American Journal of Medicine, 1987